Proteolytic substructure of brain myosin.
نویسندگان
چکیده
منابع مشابه
Pyrophosphate binding to and adenosine triphosphatase activity of myosin and its proteolytic fragments. Implications for the substructure of myosin.
The binding of pyrophosphate to myosin, heavy meromyosin, and Subfragment 1 was studied by an equilibrium dialysis technique. Myosin binds approximately 2 (1.82) moles/5 X lo5 g of protein, K = 2.07 X 10”. Identical results were obtained with myosin prepared by the ammonium sulfate precipitation procedure with or without LiCl and the dilution method. Red and white skeletal muscle myosin bound t...
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Light meromyosin (LMM), prepared by limited tryptic digestion of myosin, usually contains several polypeptide chains, LMM-A, LMM-B, and LMM-C in decreasing order of molecular weight estimated from sodium dodecyl sulfate-gel electrophoresis. Further limited tryptic digestion of LMM produces well defined fragments (Balint, M., Szilagyi, L., Fekete, Gy., Blazso, M., and Biro, E. N. A. J. Mol. Biol...
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Laser Raman spectroscopy is used to probe the heterogeneous substructure of the large contractile protein myosin. Some peaks are assigned to specific chemical groups of the molecule; others, notably the conformationally sensitive amide III vibrations, provide information on the structurally distinct regions of the molecule. Deuteration of the NH groups is instrumental in the assignment of these...
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The general topography of the myosin molecule has been elucidated by electron microscopy of shadow-cast preparations.'-3 The model obtained from these data is that of a globular head, about 200 A in diameter by 50 A high, attached to a rodlike tail 1300 to 1400 A long by 20 A wide. This picture of the myosin molecule had been anticipated on the basis of physicochemical and X-ray diffraction stu...
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Native myosin filaments from scallop striated muscle fray into subfilaments of approximately 100-A diameter when exposed to solutions of low ionic strength. The number of subfilaments appears to be five to seven (close to the sevenfold rotational symmetry of the native filament), and the subfilaments probably coil around one another. Synthetic filaments assembled from purified scallop myosin at...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1985
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)89685-5